Bacterial protein secretion is the key virulence mechanism of symbiotic and pathogenic bacteria. Thereby effector proteins are transported from the bacterial cytosol into the extracellular medium or directly into the eukaryotic host cell.
The Effective portal provides precalculated predictions on bacterial effectors in all publicly available pathogenic and symbiontic genomes as well as the possibility for the user to predict effectors in own protein sequence data.

Effector prediction in bacterial protein-sequences:

1. Provide query protein-sequence(s):

Input protein sequence(s) in multi-fasta format:

Load example data Clear  [format]

Upload a file in multi-fasta format:

2. Configure SignalP prediction:

Perform SignalP prediction

Select the organism type: gram- gram+

3. Configure EffectiveT3 prediction:

Perform EffectiveT3 prediction

Select a classification module:
Type III Effector prediction with plant set Type III Effector prediction with animal set Type III Effector prediction with standard set  [classification]

Choose cut-off setting: sensitive selective  [cutoff]
or define own cut-off (between 0 and 1.0):

4. Configure identification of eukaryotic-like domains:

Perform identification of eukaryotic-like domains

Define the minimal domain score (min. 4; to be reached in at least one pathogen genome): 
 [domain score]


  [calculation time]